St Neots Town are 7/10 favourites to win the opening day fixture next Saturday with the first bookmaker to quote odds.
Betway offer 13/5 on Hereford United winning the tie, only fractionally ahead of the draw at 3/1.
St Neots beat Conference South side Hayes & Yeading 2-0 in their away friendly match on Saturday, and are scheduled to face Peterborough's League One squad on Tuesday night. Their two latest signings, striker Nathan Frater and full back Mason Spence, both have experience of Southern League Premier football.
Hereford United warm up for the tie with a home game against a third tier County Saturday amateur league side.