Lewes, which is near Brighton, are 7th in Isthmian League Premier Division, while Crawley-based Three Bridges play in the league below in Isthmian League South East Division, and are 11th.
Lewes scored in the last minute on Saturday to take the tie to a replay, which will be played tonight.
Match highlights: www.youtube.com/watch?v=rg0OKNUuJhA
The third qualifying round is on Saturday, October 1.