The Premier League will help The National League prepare for, and deliver, the end-of-season play-offs.
Chief Executive Michael Tattersall commented: "We are pleased to receive the generous donation by the Premier League towards our end-of-season play-offs".
Premier League Chief Executive Richard Masters said: "The Premier League is a big supporter of the wider game and we will continue our commitments in this area despite our significant financial losses and ongoing uncertainty.
"We will provide £200,000 to The National League to help them to conclude their season."